Ranch error: sequence without chain ID found

[Lisa-Casteller]

Hi all,

I'm having issues setting up ranch. I keep getting this error:

error: assignment.txt: sequence without chain ID found, must be single letter A-Z

Using the following input:

ranch --repetitions 10000 --prefix pool/pep_ sequence.fasta assignment.txt

and input files:

cat assignment.txt
A 1 551 disordered

sequence.fasta
"> A
ALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPAQPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRDTRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTADEQQAPRRERSRRRNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAVVAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRSRRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQVEEQREQEEVHVQPMVTEVPVAAAIEPVVSAPVVEEVAGVVEAPVQVAEPQPEVVETTHPEVIAAAVTEQPQVITESDVAVAQEVAEQAEPVVEPQEETADIEEVVETAEVVVAEPEVVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"

I have used 'od -c' and 'echo -n "A 1 551 disordered" > assignment.txt' to make sure there weren't any hidden characters, but no matter what I do, the A does not seem to be recognised.

How should I proceed?

Thank you,
all

[maxim-biosaxs]

Please try to run the application in this way:
ranch --repetitions 10000 --prefix pool/pep_ assignment.txt sequence.fasta

[Lisa-Casteller]

Thank you, it works now!