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CTFFIND can process tiff images. Relion launches CTFFIND and executes correctly, but the ctffind_runner fails with warning messages:
WARNING: skipping, since cannot get CTF values for images/4_bl.000.tif
The issue is cttfind_runner is hard-coded to strip ".mrc" off the image filenames when looking for ctf files, so it cannot find them. For the above example, instead of looking for the 4_bl.000.ctf that ctffind created, it is looking for 4_bl.000.tif.ctf which doesn’t exist. See ctffind_runner.cpp line 252: FileName fn_microot = fn_mic_ctf_given_all[imic].without(".mrc");
and line 431: FileName fn_microot = fn_micrographs_ctf_all[imic].without(".mrc")
replacing these lines with FileName fn_microot = fn_mic_ctf_given_all[imic].withoutExtension();
and FileName fn_microot = fn_micrographs_ctf_all[imic].withoutExtension();
respectively, appears to resolve the problem with tiff images.
The text was updated successfully, but these errors were encountered:
CTFFIND can process tiff images. Relion launches CTFFIND and executes correctly, but the ctffind_runner fails with warning messages:
The issue is cttfind_runner is hard-coded to strip ".mrc" off the image filenames when looking for ctf files, so it cannot find them. For the above example, instead of looking for the 4_bl.000.ctf that ctffind created, it is looking for 4_bl.000.tif.ctf which doesn’t exist. See ctffind_runner.cpp line 252:
FileName fn_microot = fn_mic_ctf_given_all[imic].without(".mrc");
and line 431:
FileName fn_microot = fn_micrographs_ctf_all[imic].without(".mrc")
replacing these lines with
FileName fn_microot = fn_mic_ctf_given_all[imic].withoutExtension();
and
FileName fn_microot = fn_micrographs_ctf_all[imic].withoutExtension();
respectively, appears to resolve the problem with tiff images.
The text was updated successfully, but these errors were encountered: