01. Downloading SRA Toolkit

NCBI SRA Toolkit

Below are the latest releases of various tools and release checksum file.

SRA Toolkit

Compiled binaries/install scripts of April 1, 2020, version 2.10.5 (Linux and MacOSX):

• CentOS Linux 64 bit architecture - non-sudo tar archive
• Ubuntu Linux 64 bit architecture - non-sudo tar archive
• Cloud - apt-get install script - for Debian and Ubuntu - requires sudo permissions
• Cloud - yum install script - for CentOS - requires sudo permissions
• MacOS 64 bit architecture
• md5 checksums

The Linux release of SRA Toolkit version 2.10.5 is designed to work within cloud compute instances on AWS and GCP. For Windows, please continue to use version 2.9.6-1 below.

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Compiled binaries of May 7, 2019, version 2.9.6-1:

• CentOS Linux 64 bit architecture
• Ubuntu Linux 64 bit architecture
• MacOS 64 bit architecture
• MS Windows 64 bit architecture
• md5 checksums

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Magic-BLAST

Magic-BLAST is a tool for mapping large next-generation RNA or DNA sequencing runs against a whole genome or transcriptome

• Magic-BLAST executables for LINUX, MacOSX, and Windows as well as the source files are available on the FTP site
• Read more about Magic BLAST on the FTP site

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Third Party Software

Builds of Third Party Software Tools with SRA support ( NGS 2.10.5 release ):

• Genome Analysis Toolkit (GATK) version 3.6-6-ngs.2.9.0 - including direct support of SRA
• HISAT2 version 2.1.0-ngs.2.10.5 - graph-based alignment of next generation sequencing reads to a population of genomes with direct support of SRA, built for Linux 64 bit architecture

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Latest Source Code

• NGS Software Development Kit – April 1, 2020, version 2.10.5 release
• NCBI VDB Software Development Kit – April 1, 2020, version 2.10.5 release
• NCBI SRA Toolkit – April 1, 2020, version 2.10.5 release
• NCBI NGS Toolkit – April 1, 2020, version 2.10.5 release

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File checksums

You may validate downloaded files with [md5 checksums] computed using md5sum -b