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bf convergence issue
naming consistency nf partial paralellization
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Functions.md

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flowchart LR
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GetGOTerms(GetGOTerms) --> IntersectWithExpressed(IntersectWithExpressed)
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PlotGoTermScores(PlotGoTermScores) --> FeaturePlotSaveGO(FeaturePlotSaveGO)
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PlotGoTermScores(PlotGoTermScores) --> GetGOTerms(GetGOTerms)
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PlotGoTermScores(PlotGoTermScores) --> AddGOScore(AddGOScore)
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GOscoreEvaluation(GOscoreEvaluation) --> PlotGoTermScores(PlotGoTermScores)
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CalculateAndPlotGoTermScores(CalculateAndPlotGoTermScores) --> FeaturePlotSaveGO(FeaturePlotSaveGO)
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CalculateAndPlotGoTermScores(CalculateAndPlotGoTermScores) --> GetGOTerms(GetGOTerms)
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CalculateAndPlotGoTermScores(CalculateAndPlotGoTermScores) --> AddGOScore(AddGOScore)
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GOscoreEvaluation(GOscoreEvaluation) --> CalculateAndPlotGoTermScores(CalculateAndPlotGoTermScores)
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GOscoreEvaluation(GOscoreEvaluation) --> .convert.GO_term.2.score(.convert.GO_term.2.score)
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GOscoreEvaluation(GOscoreEvaluation) --> CalcClusterAverages_Gruffi(CalcClusterAverages_Gruffi)
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FilterStressedCells(FilterStressedCells) --> .convert.GO_term.2.score(.convert.GO_term.2.score)
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AddGOGeneList.manual(AddGOGeneList.manual) --> IntersectWithExpressed(IntersectWithExpressed)
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AddCustomScore(AddCustomScore) --> .fix.metad.colname.rm.trailing.1(.fix.metad.colname.rm.trailing.1)
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Shiny.GO.thresh(Shiny.GO.thresh) --> PlotNormAndSkew(PlotNormAndSkew)
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Auto.GO.thresh(Auto.GO.thresh) --> PlotNormAndSkew(PlotNormAndSkew)
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FindThresholdsShiny(FindThresholdsShiny) --> PlotNormAndSkew(PlotNormAndSkew)
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FindThresholdsAuto(FindThresholdsAuto) --> PlotNormAndSkew(PlotNormAndSkew)
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PlotNormAndSkew(PlotNormAndSkew) --> CalcStandDevSkewedDistr(CalcStandDevSkewedDistr)
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GetAllGOTermNames(GetAllGOTermNames) --> .convert.score.2.GO_term(.convert.score.2.GO_term)
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Custom gene-set derived score evaluation for filtering.
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- #### 10 `Shiny.GO.thresh()`
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- #### 10 `FindThresholdsShiny()`
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Launch Shiny App for GO Term-based Thresholding. Launches a Shiny application to interactively apply GO term-based thresholding for identifying stressed cells in a Seurat object. This function calculates the "granule average GO-scores" for individual GO terms and updates the object with a combined stress identification based on these thresholds.#'
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- #### 11 `Auto.GO.thresh()`
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- #### 11 `FindThresholdsAuto()`
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Automated GO Term-based Thresholding without using a Shiny app.. Automatically applies granular GO term-based thresholding to identify stressed cells in a Seurat object without launching a Shiny app. This function calculates the "granule average GO-scores" for individual GO terms and updates the object with a combined stress identification based on these thresholds.
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- #### 12 `FilterStressedCells()`
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Filter Stressed Cells from a Seurat Object. Identifies and filters stressed cells based on specified GO terms and a quantile threshold. It supports optional plotting of exclusion results and saving of modified datasets.
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- #### 13 `PlotGoTermScores()`
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- #### 13 `CalculateAndPlotGoTermScores()`
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Plot GO-term scores.
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NAMESPACE

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export(AddGOGeneList.manual)
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export(AddGOScore)
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export(AssignGranuleAverageScoresFromGOterm)
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export(Auto.GO.thresh)
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export(FindThresholdsAuto)
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export(AutoFindGranuleResolution)
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export(CalcClusterAverages_Gruffi)
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export(CalcStandDevSkewedDistr)
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export(GrScoreUMAP)
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export(IntersectWithExpressed)
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export(PlotClustSizeDistr)
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export(PlotGoTermScores)
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export(CalculateAndPlotGoTermScores)
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export(PlotNormAndSkew)
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export(ReassignSmallClusters)
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export(Shiny.GO.thresh)
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export(FindThresholdsShiny)
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export(UMAP3Dcubes)
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importFrom(AnnotationDbi,Term)
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importFrom(AnnotationDbi,select)

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