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TetradAnalysis

Background

This program is intended to be used as a tool to aid in counting pollen tetrads in three color fluorescent intervals (fluorescent tagged lines, or FTLs). The user is expected to either have 1) image data in three colors (e.g., CFP, YFP, and dsRED) or 2) a slide with three-color pollen tetrads on a epifluorescence microscope.

For the original description of the FTLs, see here:

Francis KE, Lam SY, Harrison BD, Bey AL, Berchowitz LE and Copenhaver GP (2007) Pollen Tetrad-based Visual Assay for Meiotic Recombination in Arabidopsis. Proc. Natl. Acad. Sci.104: 3913-3918. doi:10.1073/pnas.0608936104

For a description of the different tetrad types, see here:

Berchowitz LE, Copenhaver GP (2008) Fluorescent Arabidopsis tetrads: A visual assay for quickly developing large crossover and crossover interference datasets. Nature Protocols 3(1): 41-50. doi:10.1038/nprot.2007.491

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